20211003 Assay Dev

Hand-in-hand with academic work, we also develop new and accessible methods for energy metabolism research. Additionally, we aim to help others design experiments and interpret data with methods-focused roadmaps and perspectives.

Related Publications

Jones AE, Arias NJ, Acevedo A, Reddy ST, Divakaruni AS*§, and Meriwether D*§ (2021) A single LC-MS/MS analysis to quantify CoA biosynthetic intermediates and short-chain acyl CoAs. Metabolites. 11(8): 468. 34436409

Yang K, Doan MT, Stiles L, and Divakaruni AS (2021) Measuring CPT-1-mediated respiration in permeabilized cells and isolated mitochondria. STAR Protocols. 2: 100687. 34401773

Divakaruni AS§, Andreyev AY, Rogers GW, and Murphy AN (2018) In situ measurements of mitochondrial matrix enzyme activities using plasma and mitochondrial membrane permeabilization agents. Analytical Biochemistry. 552:60-65. 28987935

Divakaruni AS, Jastroch M. (2022) A practical guide for the analysis, standardization and interpretation of oxygen consumption measurements. Nature Metabolism. 4, 978-994

DeSousa BR, Kim KO, Jones AE, Ball AB, Hsieh WY, Swain P, Morrow DH, Brownstein A, Ferrick D, Shirihai OS, Neilson A, Nathanson D, Rogers G, Drank B, Murphy A, Affourtit C, Bensinger SJ, Stiles L, Romero N, Divakaruni AS (2023) Calculation of ATP production rates using the Seahorse XF Analyzer EMBO Reports. 24(10):e56380 37548091