20211003 Assay Dev

Hand-in-hand with academic work, we also develop new and accessible methods for energy metabolism research. Additionally, we aim to help others design experiments and interpret data with methods-focused roadmaps and perspectives.

Related Publications

Jones AE, Arias NJ, Acevedo A, Reddy ST, Divakaruni AS*§, and Meriwether D*§ (2021) A single LC-MS/MS analysis to quantify CoA biosynthetic intermediates and short-chain acyl CoAs. Metabolites. 11(8): 468. 34436409

Yang K, Doan MT, Stiles L, and Divakaruni AS (2021) Measuring CPT-1-mediated respiration in permeabilized cells and isolated mitochondria. STAR Protocols. 2: 100687. 34401773

Divakaruni AS§, Andreyev AY, Rogers GW, and Murphy AN (2018) In situ measurements of mitochondrial matrix enzyme activities using plasma and mitochondrial membrane permeabilization agents. Analytical Biochemistry. 552:60-65. 28987935

Divakaruni AS, Jastroch M. (2022) A practical guide for the analysis, standardization and interpretation of oxygen consumption measurements. Nature Metabolism. 4, 978-994